Good afternoon everyone. So this is week 2 of S-STEM and I have learned very much about the procedures and how to classify bacteria. This week I took my TSA plates and saw that bacteria had multiplied. By this I was able to choose one single colony to begin my procedures in finding what kind of bacteria it is. Along the way, I made a simple error and unfortunately I have to redo it again. It was after putting them in the micro glass that adding the chemicals to help identify the characteristics of bacteria is where I made an error as. I noticed when I saw through them on the microscope that I couldn't identify them as either gram negative or gram positive because they were both colors. As a result, yesterday I began a new TSA plate and let it incubate for 24hrs. However, coming back today, I took it out and saw that nothing had grown on it! So I have redone now two TSA plates: One using the broth and the other using the actual dish that the bacteria was on. Hopefully by tomorrow I have the multiplied growth of bacteria to find my unknown.
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| Multiplied growth of unknown Bacteria |
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| Micro glasses with my bacteria to view under a microscope |
Hey what's up Oliver,
ReplyDeleteThat's so strange that you had nothing grow on your TSA plate. I remember doing this project when I started I thought it was so much fun doing all the different type of procedures to single down the type of bacteria you have. I wonder what could have gone wrong with your TSA plate to show no growth of bacteria at all. Hopefully something grows by tomorrow so you can go on with the experiment. It's pretty cool how the test can help you classify the bacteria. Let's just hope you come down to the right one haha.