This week we poured and ran the gels for our positive
controls, ran PCR on our positive controls and screened the positive controls
to choose the best ones to create more positive controls to test the
Pseudomonas (PA) and (PS) control samples. The PA samples are those of
Pseudomonas aeruginosa and the PS samples of Pseudomonas species. We also
learned how to do DNA extraction from the gels by cutting the bands out which
is something new that I hadn’t done before nor did I perform this on my last
project working with the protocols for E. coli. So definitely it was fun
learning a new procedure that can be done to keep on confirming our DNA and
positive controls.
| Our tubes needed to perform PCR containing the primers. |
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